Molecular cloning and nucleotide sequence of Thermus thermophilus HB8 trpE and trpG |
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Authors: | Showbu Sato Yukari Nakada Shigenori Kanaya Teruo Tanaka |
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Abstract: | The trpE gene of Thermus thermophilus HB8 was cloned by complementation of an Escherichia coli tryptophan auxotroph. The E. coli harboring the cloned gene produced the anthranilate synthase I, which was heat-stable and enzymatically active at higher temperature. The nucleotide sequence of the trpE gene and its flanking regions was determined. The trpE gene was preceded by an attenuator-like structure and followed by the trpG gene, with a short gap between them. No other gene essential for tryptophan biosynthesis was observed after the trpG gene. The amino-acid sequences of the T. themophilus anthranilate synthase I and II deduced from the nucleotide sequence were compared with those of other organisms. |
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Keywords: | trpE trpG Nucleotide sequence Anthranilate synthase Molecular cloning (T. thermophilus HB8) |
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