Neutrophil depletion retards endometrial repair in a mouse model |
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Authors: | Tu’uhevaha J Kaitu’u-Lino Naomi B Morison Lois A Salamonsen |
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Institution: | (1) Level 4, Block E, Monash Medical Centre, Prince Henry’s Institute of Medical Research, 246 Clayton Road, Clayton, Victoria, 3168, Australia;(2) Prince Henry’s Institute of Medical Research, P.O. Box 5152, Clayton, Victoria, Australia;(3) Department of Obstetrics and Gynecology, Monash University, Clayton, Victoria, 3168, Australia |
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Abstract: | The contribution of the high abundance of inflammatory cells present in the human endometrium prior to and during menstruation
is unknown with respect to endometrial repair and/or menstruation. In this study, the presence and localisation of markers
for key inflammatory cells have been examined in a mouse model of endometrial breakdown and repair and the functional contribution
of neutrophils has been determined. In the model, decidualisation is artificially induced and progesterone support withdrawn;
the endometrial tissue progressively breaks down by 24 h after progesterone withdrawal and, by 48 h, has usually undergone
complete repair. Neutrophils have been identified in low abundance in decidual tissue, rise in number during breakdown and
are most abundant during early repair. Macrophages are barely detectable during breakdown or repair in this model, whereas
uterine natural killer cells are found only in intact decidua. The functional contribution of neutrophils to endometrial breakdown
and repair has been assessed via neutrophil depletion by using the antibody RB6-8C5. This antibody significantly depletes
neutrophils from the circulation and tissue, affects endometrial breakdown and markedly delays endometrial repair. This study
has therefore demonstrated that neutrophils are the most abundant leucocyte in this model and that they play an important
functional role in the processes of endometrial breakdown and repair.
This work was funded by the National Health and Medical Research Council of Australia (#143798, #241000) and by an Australian
Postgraduate Scholarship to T.K. |
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Keywords: | Uterus Endometrium Neutrophils Menstruation Mouse model (C57BL/6) |
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