Deletogenic activity of 1,2:7,8-diepoxyoctane in the Salmonella typhimurium tester strain TA102. |
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| Authors: | J N Picada N N Khromov-Borisov J A Henriques |
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| Institution: | Departamento de Biofsica, GENOTOX-Laboratório de Genotoxicidade/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Bloco IV, Prdio 43.421, Caixa Postal 15.005, Campus do Vale/UFRGS, CEP 91501-970, Porto Alegre, RS, Brazil. |
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| Abstract: | 1,2:7,8-Diepoxyoctane (DEO), whose deletogenic activity was first demonstrated in ad-3 system of Neurospora crassa and then in different species, has been tested in Salmonella typhimurium tester strain TA102 (hisG428(Ochre)). It was confirmed that it is a direct acting mutagen and was found that its activity is stimulated with the S9 mix. Obtained His(+) revertants were screened on their response to the histidine analog, N-(2-thiazolyl)-DL-alanine (ThiAla). Thirty-two percent of spontaneous and 52% of DEO-induced revertants were resistant to the analog while no resistance was observed among those induced with 4-nitroquinoline-N-oxide (4NQO). Resistance to ThiAla was interpreted as due to small deletions surrounding the target TAA codon in hisG428(Ochre). Thus, at least two simple test-systems, ad-3 of N. crassa and hisG428(Ochre) of S. typhimurium, gave compatible results and might be useful in searching of deletogens. |
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