| 流式细胞术检测胃癌前病变DNA及p21，p53含量 |
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| 引用本文： | 齐凤英,段惠军,刘庆银,左连富,刘江惠,郭建文. 流式细胞术检测胃癌前病变DNA及p21，p53含量[J]. 生物化学与生物物理进展, 1998, 25(4): 363-366 |
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| 作者姓名： | 齐凤英 段惠军 刘庆银 左连富 刘江惠 郭建文 |
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| 作者单位： | 河北医科大学病理解剖教研室，石家庄 050017;河北医科大学病理解剖教研室，石家庄 050017;河北医科大学病理解剖教研室，石家庄 050017;河北省肿瘤研究所，石家庄 050011;河北省肿瘤研究所，石家庄 050011;河北省肿瘤研究所，石家庄 050011 |
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| 基金项目： | 河北省卫生厅资助项目(94001). |
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| 摘 要： | 应用流式细胞术和免疫荧光技术,对80例胃癌前病变细胞中DNA含量，rasp21和p53蛋白进行定量检测,探讨其在胃癌前病变中,作为癌变早期标志物的临床意义.检测结果发现,胃癌前病变的DNA异倍体率随不典型增生病变的分级增高而增加, rasp21和p53蛋白的表达量亦随胃癌前病变不典型增生程度的加重而增高.DNA异倍体和rasp21表达阳性的胃癌前病变出现的癌变率显著增高,提示可能是癌变早期的分子标志物.
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| 关 键 词： | 胃癌前病变，DNA含量，流式细胞术 |
| 收稿时间： | 1997-06-10 |
| 修稿时间： | 1997-10-24 |
Detection of DNA and Content of p21 and p53 for Gastric Precancer Lesion by Flow Cytometry |
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| QI Feng-ying,DUAN Hui-jun,LIU Qing-yin,ZUO Lian-fu,LIU Jiang-hui and GUO Jian-wen. Detection of DNA and Content of p21 and p53 for Gastric Precancer Lesion by Flow Cytometry[J]. Progress In Biochemistry and Biophysics, 1998, 25(4): 363-366 |
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| Authors: | QI Feng-ying DUAN Hui-jun LIU Qing-yin ZUO Lian-fu LIU Jiang-hui GUO Jian-wen |
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| Affiliation: | Department of Pathology Hebei Medical University, Shijiazhuang 050017, China;Department of Pathology Hebei Medical University, Shijiazhuang 050017, China;Department of Pathology Hebei Medical University, Shijiazhuang 050017, China;Hebei Provincial tumor Institute, Shijiazhuang 050011,China;Hebei Provincial tumor Institute, Shijiazhuang 050011,China;Hebei Provincial tumor Institute, Shijiazhuang 050011,China |
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| Abstract: | Content of DNA, rasp21 and p53 in 80 case gastric precancer lesion was quantitative detected by flow cytometry and immunofluorescence techniques. and to investigate its in clinical value as early carceration marker.The detected results showed that raising in DNA heteroploid rate. Rasp21 and p53 protein expression were found with raising of dysplasia grade in gastric precancer lesion. The rate of canceration of gastric precancer lesionin in DNA heteroploid and rasp21 expression positive were notable increased. DNA heteroploid and rasp21 protein over-expression can be an early canceration molecular marker. |
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| Keywords: | gastric precancer lesion DNA content flow cytometry |
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