| 头孢菌素酰化酶 |
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| 引用本文： | 李勇,王恩多. 头孢菌素酰化酶[J]. 生物化学与生物物理进展, 1998, 25(3): 238-242 |
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| 作者姓名： | 李勇 王恩多 |
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| 作者单位： | 中国科学院上海生物化学研究所, 分子生物学国家重点实验室, 上海 200031;中国科学院上海生物化学研究所, 分子生物学国家重点实验室, 上海 200031 |
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| 基金项目： | 国家“863”计划资助项目. |
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| 摘 要： | 7-氨基头孢烷酸(7-amino cephalosporanic acid, 7-ACA)是医药工业合成大多数头孢菌素的重要原料.头孢菌素酰化酶(cephalosporin acylase, CA)催化头孢菌素C(CPC)和戊二酰-7-氨基头孢烷酸(GL-7ACA)的水解反应, 生成7-ACA.根据CA催化底物的不同, 可将其划分为两类：CPC酰化酶和GL-7ACA酰化酶.由CA的同源性、分子质量大小和基因结构, 可以把头孢菌素酰化酶划分为五种；讨论了酶的基本性质.通过CA与N端亲核水解酶(Ntn水解酶)的比较, 推测CA属于Ntn水解酶, 并由此可以进一步理解它们的生理功能.
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| 关 键 词： | 头孢菌素酰化酶 7-氨基头孢烷酸 N端亲核水解酶 |
| 收稿时间： | 1997-03-27 |
| 修稿时间： | 1997-09-05 |
Cephalosporin Acylases |
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| LI Yong and WANG En-duo. Cephalosporin Acylases[J]. Progress In Biochemistry and Biophysics, 1998, 25(3): 238-242 |
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| Authors: | LI Yong and WANG En-duo |
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| Affiliation: | State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, The Chinese Academy of Sciences, Shanghai 200031, China;State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, The Chinese Academy of Sciences, Shanghai 200031, China |
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| Abstract: | Cephalosporin acylases (CA) are enzymes that catalyze hydrolysis of the acyl side chain of cephalosporin C(CPC) or glutaryl 7-cephalosporanic acid(GL-7ACA) to yield 7-aminocephalosporanic acid(7-ACA), the most important compound in the production of many semisynthetic cephalosporin antibiotics. According to their substrates, cephalosporin acylases can be classified into two classes: CPC acylase and GL-7ACA acylase. CA was divided into five types on the basis of their homologies and mass of molecules, and the structures of their genes; some properties of these enzymes were discussed. Comparison of N-terminal nucleophile hydrolases and CA shows that CA may belong to N-terminal nucleophile hydrolases family. Thus it makes easy to understand further their physiological roles. |
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| Keywords: | cephalosporin acylases 7-aminocephalosporinic acid N-terminal nucleophile hydrolases |
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