| 多彩色荧光原位杂交技术原理及其应用 |
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| 引用本文： | 杨明杰,曹佳.多彩色荧光原位杂交技术原理及其应用[J].生物化学与生物物理进展,1998,25(4):333-337. |
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| 作者姓名： | 杨明杰 曹佳 |
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| 作者单位： | 第三军医大学预防医学系分子毒理学实验室，重庆 400038;第三军医大学预防医学系分子毒理学实验室，重庆 400038 |
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| 摘 要： | 多彩色荧光原位杂交是一门新兴的分子细胞遗传学技术，它用几种不同颜色的荧光素单独或混合标记的探针，进行原位杂交，同时检测间期细胞或中期细胞中的几个特异核酸序列，为分析癌症遗传不稳定性提供了一种简便、快速、可靠的方法，并广泛应用于物理图谱绘制、致突变研究、肿瘤病理学和产前诊断.
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| 关 键 词： | 多彩色荧光原位杂交 染色体描绘 多彩色原位启动标记 比较基因组杂交 |
| 收稿时间： | 1997/5/23 0:00:00 |
| 修稿时间： | 1997/9/20 0:00:00 |
Principle and Applications of Multicolor Fluorecsence in situ Hybridization Technology |
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| YANG Ming-jie and CAO Jia.Principle and Applications of Multicolor Fluorecsence in situ Hybridization Technology[J].Progress In Biochemistry and Biophysics,1998,25(4):333-337. |
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| Authors: | YANG Ming-jie and CAO Jia |
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| Institution: | Molecular Toxicology Laboratory, Third Military Medical College,Chongqing 400038, China;Molecular Toxicology Laboratory, Third Military Medical College,Chongqing 400038, China |
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| Abstract: | Multicolor fluorescence in situ hybridization, a new promising molecular cytogenetic technique, permits the simultaneous detection several specific nucleic acid sequences in interphase as well as metaphase by using cohybridization of probes labeled with serveral fluorescent labels or label combinations. It provids a simple, fast, and reliable means to assess genetic instability in cancer. This approach is being applied generally in physical mapping,mutation research, tumor pathology and prenatal dignosis. |
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| Keywords: | multicolor fluorescence in situ hybridization chromosome painting multicolor primed in situ labeling comparative genomic hybridization |
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