| 噬菌体DNA的快速抽提 |
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| 引用本文： | 覃文新,万大方,赵新泰,蒋惠秋,顾健人.噬菌体DNA的快速抽提[J].生物化学与生物物理进展,1998,25(2):186-187. |
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| 作者姓名： | 覃文新 万大方 赵新泰 蒋惠秋 顾健人 |
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| 作者单位： | 上海市肿瘤研究所癌基因及相关基因国家重点实验室，上海 200032;上海市肿瘤研究所癌基因及相关基因国家重点实验室，上海 200032;上海市肿瘤研究所癌基因及相关基因国家重点实验室，上海 200032;上海市肿瘤研究所癌基因及相关基因国家重点实验室，上海 200032;上海市肿瘤研究所癌基因及相关基因国家重点实验室，上海 200032 |
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| 摘 要： | 介绍一种噬菌体DNA的快速抽提方法.用聚乙二醇沉淀噬菌体颗粒，然后经DEAE纤维素纯化处理和酚抽提.与传统的噬菌体DNA纯化方法相比，改进后的方法方便、快速、经济，可获得高纯度的噬菌体DNA.
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| 关 键 词： | 噬菌体DNA，抽提，聚乙二醇，DEAE纤维素 |
| 收稿时间： | 1997/3/20 0:00:00 |
| 修稿时间： | 1997/7/12 0:00:00 |
Rapid Extraction of Phage DNA |
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| QIN Wen-xin,WAN Da-fang,ZHAO Xin-tai,JIANG Hui-qiu and GU Jian-ren.Rapid Extraction of Phage DNA[J].Progress In Biochemistry and Biophysics,1998,25(2):186-187. |
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| Authors: | QIN Wen-xin WAN Da-fang ZHAO Xin-tai JIANG Hui-qiu and GU Jian-ren |
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| Institution: | National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China;National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China;National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China;National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China;National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China |
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| Abstract: | A purification method for phage DNA is introduced. Phage particles are precipitated by polyethylene glycol (PEG), then purified by DEAE-cellulose DE52 and extracted by phenol. This modified method is more convenient, rapid and economical than the traditional method of phage DNA purification and can obtain phage DNA with high purity. |
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| Keywords: | phage DNA extraction polyethylene glycol (PEG) DEAE-cellulose DE52 |
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