| 一种改进的分离嗜中性白细胞的方法 |
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| 引用本文： | 贝泠,胡天惠,沈恂. 一种改进的分离嗜中性白细胞的方法[J]. 生物化学与生物物理进展, 1998, 25(4): 359-363 |
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| 作者姓名： | 贝泠 胡天惠 沈恂 |
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| 作者单位： | 中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101 |
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| 基金项目： | 国家自然科学基金资助项目(39670205). |
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| 摘 要： | 报道一种从全血中分离嗜中性白细胞的方法.人血经过葡聚糖沉降，淋巴细胞分离液梯度分离，特殊分离液的洗涤和红细胞的溶胀后，得到了嗜中性白细胞.经瑞特氏染色和台盼蓝染色证明细胞纯度与存活率均在95％以上.以化学发光和细胞色素c还原法证明了细胞的高活力与膜受体的完整性.与国外的分离方法相比较，该方法简便易行，且分离效果好，是一种高效、经济的分离方法.
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| 关 键 词： | 嗜中性白细胞， 分离提纯， 呼吸爆发 |
| 收稿时间： | 1997-05-28 |
| 修稿时间： | 1997-09-15 |
A Modified Method for Seperating Neutrophils From Human Blood |
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| BEI Ling,HU Tian-hui and SHEN Xun. A Modified Method for Seperating Neutrophils From Human Blood[J]. Progress In Biochemistry and Biophysics, 1998, 25(4): 359-363 |
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| Authors: | BEI Ling HU Tian-hui SHEN Xun |
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| Affiliation: | Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | A modified method for seperating neutrophils from whole blood was reported. Neutrophils was obtained by dextran sedimentation, centrifuged with lymphocytes separating solution, special seperting medium washing and erythrocyte depletion. The purity and viability were proved to be both about 95% by Wright's stain and Typan blue respectively. Both the chemiluminescence method and cytochrom c reduction method proved the high activity and the integrity of the membrane receptor of the neutrophils. Compared with other method, this method is more simple and easier to handle, which suggests that it is a efficient and economic method for seperating neutrophils. |
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| Keywords: | neutrophil seperation respiratory burst |
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