| 人CD14基因的克隆及序列测定 |
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| 引用本文： | 龙建银,薛沿宁,孙蕾,王会信.人CD14基因的克隆及序列测定[J].生物化学与生物物理进展,1998,25(4):377-378. |
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| 作者姓名： | 龙建银 薛沿宁 孙蕾 王会信 |
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| 作者单位： | 军事医学科学院基础医学研究所，北京 100850;军事医学科学院基础医学研究所，北京 100850;军事医学科学院基础医学研究所，北京 100850;军事医学科学院基础医学研究所，北京 100850 |
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| 摘 要： | 采用PCR技术，从佛波酯乙醇刺激的HL-60细胞基因组DNA中扩增获得了1.1 kb的人CD14基因.序列分析表明，有四个碱基发生了突变，其中两处为首次报道.
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| 关 键 词： | CD14，脂多糖，基因克隆 |
| 收稿时间： | 2/6/1998 12:00:00 AM |
| 修稿时间： | 1998/4/21 0:00:00 |
Cloning and Sequencing of Human CD14 Gene |
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| LONG Jian-yin,XUE Yan-ning,SUN Lei and WANG Hui-xin.Cloning and Sequencing of Human CD14 Gene[J].Progress In Biochemistry and Biophysics,1998,25(4):377-378. |
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| Authors: | LONG Jian-yin XUE Yan-ning SUN Lei and WANG Hui-xin |
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| Institution: | Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China |
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| Abstract: | Human CD14 gene was cloned from genomic DNA of PMA-stimulated HL-60 cells by PCR. Sequencing result showed that it is identical to the reported human CD14 gene except four bases, among which two new mutations were identified. |
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| Keywords: | CD14 lipopolysaccharide gene cloning |
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