数据综合分析鉴定人类Auxilin基因

Auxilin蛋白诱导Hsp70c蛋白与笼形蛋白的结合，在真核细胞衣被小泡脱衣被的过程中扮演了重要的角色.通过对已有EST，STS等数据库的综合分析，我们将人类auxilin基因定位到1p31,D1S515和D1S198标记之间.26个EST构成的5个重叠群，占该基因中共约2.3 kb的部分cDNA序列，其中编码区长501 bp，得到的序列与牛的auxilin基因显示有极高的同源性.各EST数据显示，auxilin在人胚胎的多种组织中表达，在成人脑、表皮组织中也有表达.

Identification and Mapping of Human Auxilin Gene by Integrated Data Analysis

Auxilin is a molecular chaperon which induces binding of Hsp70c to clathrin, playing an important role in uncoating of coated vesicle isolated from brain. Through integrated analysis of public database such as dbEST, dbSTS, partial cDNA sequence of human auxilin was identified and mapped to 1p31, between marker D1S515 and D1S198. Totally 26 ESTs were found to be part of human auxilin and used to construct five contigs, which make totally 2.3 kb sequence and contains 501 bp coding sequence. All the sequences obtained and the corresponding putative translation showed high homologous to bovine auxilin. At the same time, EST data indicate that human auxilin expressed in several tissues at fetal stage, also in brain and melanocyte at adult stage.