Effect of oxotremorine,physostigmine, and scopolamine on brain acetylcholine synthesis: A study using HPLC |
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Authors: | N Bertrand A Beley |
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Institution: | (1) Laboratoire de Pharmacodynamie et de Physiologie Pharmaceutique, Faculté de Pharmacie, 7 bd Jeanne d'Arc, 21033 Dijon, Cedex, France |
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Abstract: | The synthesis rate of brain acetylcholine (ACh) was estimated in mice following i.v. administration of 3H]choline (Ch). The measurements were performed 1 min after the tracer injection, using the 3H]ACh/3H]Ch specific radioactivity ratio as an index of ACh synthesis rate. Endogenous and labeled Ch and ACh were quantified using HPLC methodology. Oxotremorine and physostigmine (0.5 mg/kg, i.p.) increased the steady state concentration of brain ACh by +130% and 84%, respectively and of Ch by +60% (oxotremorine); they decreased ACh synthesis by 62 and 55%, respectively. By contrast, scopolamine (0.7 mg/kg, i.p.) decreased the cerebral content of Ch by –26% and of ACh by –23% without enhancing the synthesis of ACh. The results show the utility of HPLC methodology in the investigation of ACh turnover. |
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Keywords: | Oxotremorine physostigmine scopolamine acetylcholine synthesis mouse brain HPLC |
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