Development, inheritance and cross-species amplification of microsatellite markers from Acacia mangium |
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Authors: | P A Butcher S Decroocq Y Gray G F Moran |
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Institution: | (1) CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU |
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Abstract: | Microsatellite markers were developed in Acacia mangium Willd. to provide highly variable co-dominant markers for linkage mapping and studies of the breeding system. After an enrichment
procedure 40% of colonies contained microsatellites in contrast with less than 1% from a non-enriched library. The majority
of microsatellite sequences were AC repeats. Co-dominant segregation of alleles in two full-sib crosses of A. mangium was demonstrated at 33 microsatellite loci. The markers were highly variable relative to restriction fragment lengths polymorphisms
(RFLPs). In the two pedigrees 53% of microsatellite loci were fully informative compared with 15% of RFLPs. Based on alleles
detected among four parental genotypes, the microsatellites consisting of dinucleotide repeats were more polymorphic than
those with tri- and tetra-nucleotide repeats. The microsatellite markers were not as transferable across species in the genus
Acacia as RFLPs. Two thirds of the primers developed in A. mangium (subgenus Phyllodineae, section Juliflorae) amplified DNA from other species within the same section but failed to amplify
in species from the subgenus Acacia. The availability of multiallelic, PCR-based, co-dominant microsatellite loci makes possible
efficient studies of gene flow and breeding systems in A. mangium, a species with low allozyme variation.
Received: 30 December 1999 / Accepted: 10 May 2000 |
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Keywords: | Acacia mangium Microsatellites Simple sequence repeats Genome mapping |
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