| 亚适温弱光对黄瓜幼苗光合酶活性和基因表达的影响 |
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| 引用本文： | Bi HG,Wang ML,Jiang ZS,Dong XB,Ai XZ. 亚适温弱光对黄瓜幼苗光合酶活性和基因表达的影响[J]. 应用生态学报, 2011, 22(11): 2894-2900 |
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| 作者姓名： | Bi HG Wang ML Jiang ZS Dong XB Ai XZ |
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| 作者单位： | 山东农业大学园艺科学与工程学院/园艺作物生物学农业部重点开放实验室,山东泰安271018;山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安271018 |
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| 基金项目： | 国家自然科学基金项目(30972032); 国家基础研究发展计划重点项目(2009CB119000)资助 |
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| 摘 要： | 以‘津优3号’为试材,研究亚适温弱光(18℃/12℃,100 μmol·m-2·s-1)下黄瓜幼苗叶片核酮糖-1,5-二磷酸羧化/加氧酶(Rubisco)、果糖-1,6-二磷酸酶(FBPase)、甘油醛-3-磷酸脱氢酶(GAPDH)、果糖-1,6-二磷酸醛缩酶(FBA)、转酮醇酶(TK) mRNA表达量及活性的变化.结果表明:亚适温弱光处理的单株叶面积和干物质量均明显减小.处理初期,Rubisco大亚基(rbcL)、小亚基(rbcS)、FBPase、GAPDH、FBA及TK的基因表达量大幅度下降,多数酶活性明显减弱(TK变化不明显),光合速率(Pn)快速降低;处理3d后,亚适温弱光处理的rbcL、rbcS基因表达量和Rubisco初始活性持续下降,但下降幅度明显减小,Rubisco总活性及FBPase、GAPDH、FBA和TK基因表达与活性均呈上升趋势,Pn同步回升;处理时间超过6d时,Rubisco和FBPase基因表达与活性趋于平稳,其他酶和Pn呈下降趋势.可见,亚适温弱光下黄瓜光合酶基因表达量和活性的降低是Pn降低的重要原因,光合机构对亚适温弱光的适应与光合酶的活化机制有关.
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| 关 键 词： | 黄瓜 酶活性 基因表达 弱光 亚适温 |
Impacts of suboptimal temperature and low light intensity on the activities and gene expression of photosynthetic enzymes in cucumber seedling leaves |
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| Bi Huan-Gai,Wang Mei-Ling,Jiang Zhen-Sheng,Dong Xu-Bing,Ai Xi-Zhen. Impacts of suboptimal temperature and low light intensity on the activities and gene expression of photosynthetic enzymes in cucumber seedling leaves[J]. The journal of applied ecology, 2011, 22(11): 2894-2900 |
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| Authors: | Bi Huan-Gai Wang Mei-Ling Jiang Zhen-Sheng Dong Xu-Bing Ai Xi-Zhen |
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| Affiliation: | BI Huan-gai1,2,WANG Mei-ling1,JIANG Zhen-sheng1,DONG Xu-bing1,AI Xi-zhen1,2(1Ministry of Agriculture Key Laboratory of Horticultural Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an 271018,Shandong,China,2State Key Laboratory of Crop Biology,China) |
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| Abstract: | Taking the cucumber cultivar 'Jinyou 3' as test material,this paper studied the variations of the mRNA expression and activities of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco),fructose-1,6-bisphosphatase(FBPase),glyceraldehydes-3-phosphate dehydrogenase(GAPDH),fructose-1,6-bisphosphate aldolase(FBA),and transketolase(TK) in cucumber seedling leaves under suboptimal temperature and low light intensity(ST+LL).In the treatment of ST+LL,the leaf area and the dry mass per plant decreased remarkably,... |
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| Keywords: | cucumber enzyme activity gene expression low light intensity suboptimal temperature. |
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