Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium |
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Authors: | Yong Kwon Lee Seung Yeul Kim Ki Heon Kim Bok-Hwan Chun Kweon-Haeng Lee Duk Jae Oh Namhyun Chung |
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Institution: | (1) College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, South Korea;(2) Department of Pharmacology, The Catholic University of Korea, Seoul, 137-701, South Korea;(3) Department of Bioscience and Biotechnology, Sejong University, Seoul, 143-747, South Korea |
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Abstract: | Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can
be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium.
Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of
viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation
(basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although
they do not fully substitute for BPE. |
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Keywords: | Soybean protein hydrolysates Cell growth Keratinocytes Serum-free medium |
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