Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’ |
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| Authors: | V Gustafson S Mallubhotla J MacDonnell M Sanyal-Bagchi B Chakravarty G Wang-Pruski C Rothwell P Audy D De Koeyer M Siahbazi B Flinn S Regan |
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| Institution: | (1) Canadian Potato Genome Project-BioAtlantech Unit Genome Atlantic, E3B 6Z9 Fredericton, NB, Canada;(2) Department of Biology, Carleton University, K1S 5B6 Ottawa, ON, Canada;(3) BioAtlantech, E3B 5A6 Fredericton, NB, Canada;(4) Department of Plant and Animal Sciences, Nova Scotia Agricultural College, B2N 5E3 Truro, NS, Canada;(5) Solanum Genomics International Inc., E3B 6Z9 Fredericton, NB, Canada;(6) Agriculture and Agri-Food Canada, E3B 4Z7 Fredericton, NB, Canada;(7) Department of Biology, Queen’s University, K7L 3N6 Kingston, ON, Canada |
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| Abstract: | As part of a large-scale genomics project focused on understanding and improving the Shepody potato, we have increased the regeneration and transformation rates for this cultivar. Using combinations of auxins and trans-zeatin, leaf and stem explants were evaluated for callus induction and shoot formation. Several plant growth regulator combinations resulted in higher plant regeneration rates over a previous method. Using the best combination of auxin and cytokinin in combination with Agrobacterium-mediated transformation, we regenerated independent putative transformants from 59.5% of the total explants plated. We ran PCR on a sample of the plants to confirm transformation and 47.1% were nptII positive; giving a confirmed transformation rate of 28.0%. |
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