Ultrasensitive DNA chip: gene expression profile analysis without RNA amplification |
| |
Authors: | Nagino Kunihisa Nomura Osamu Takii Yuki Myomoto Akira Ichikawa Makiko Nakamura Fumio Higasa Masashi Akiyama Hideo Nobumasa Hitoshi Shiojima Satoshi Tsujimoto Gozoh |
| |
Institution: | New Frontiers Research Laboratories, Toray Industries, Inc., Kanagawa 248-8555. Kunihisa_nagino@nts.toray.co.jp |
| |
Abstract: | We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|