Comparative studies on cell lines established from normal and radiation-exposed miniature swine |
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Authors: | M E Frazier R N Ushijima T K Andrews M J Hooper |
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Institution: | (1) Battelle, Pacific Northwest Laboratory, Operated by Battelle Memorial Institute, 99352 Richland, Washington;(2) Present address: Department of Microbiology, University of Montana, Missoula, Montana |
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Abstract: | Summary Cloned cell lines were established from two swine with radiation-induced myeloproliferative disorders, including one cell
culture from an animal with myelogenous leukemia and one from an animal with myeloid metaplasia. A third cloned cell line
with similar morphology was established from pooled normal fetal swine cornea to compare the growth characteristics of cells
from normal and irradiated swine. All three cell lines grew as foci of aggregated cells and were able to form macroscopic
colonies in semisolid agar medium. The lack of normal mechanisms of contact inhibition and the observed aneuploidy indicated
that these cells were morphologically transformed. Further, the cloned cells caused tumors in nude mice, clearly indicating
that these cells were also malignantly transformed. A major difference between these cell lines was that type C viruses were
observed only in the cells derived from swine with myeloproliferative disorders.
Work supported by the U.S. Department of Energy under Contract EY-76-C-06-1830. |
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Keywords: | chromosomes growth characteristics leukemia metaplasia miniature swine radiation |
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