DNA-Induced Aggregation and Fusion of Phosphatidylcholine Liposomes in the Presence of Multivalent Cations Observed by the Cryo-TEM Technique |
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Authors: | Vasily V Kuvichkin Radostin S Danev Hideki Shigematsu Kuniaki Nagayama |
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Institution: | (1) Department of Mechanisms Reception of the Institute of Cell Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Reg., Russia;(2) Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1, Higashiyama, Myodaiji-cho, Okazaki 444-8787, Japan |
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Abstract: | By means of cryoelectron transmission microscopy (cryo-TEM), we were able to demonstrate the formation of ternary complexes
(TC): DNA–phosphatidylcholine liposome–divalent metal cations. Addition of Ba2+ to TC led to visualization of DNA compacting on the liposome surface. Staining the TC by Tb3+ cations revealed the changed secondary structure of DNA located between fused liposomes. Cryo-TEM and liposome turbidity
data were analyzed during TC formation. Liposome aggregation and the liposome fusion induced by DNA in TC were observed. Because
TC displayed the property of DNA cationic liposome complexes as well as their own unique properties, we were able to consider
cationic lipoplexes as a particular case of TC. The involvement of TC and direct DNA–lipid interactions in the formation nuclear
pore complexes were assumed. |
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Keywords: | DNA Phosphatidylcholine liposome Metal cation Liposome fusion Cryo-TEM |
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