Infection and development of Nosema bombycis (Microsporida: Protozoa) in a cell line of Antheraea eucalypti |
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Authors: | Takeshi Kawarabata Ren Ishihara |
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Institution: | Institute of Biological Control, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812, Japan;Faculty of Agriculture and Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252, Japan |
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Abstract: | Spores of Nosema bombycis derived from diseased insects were highly purified by Urografin density gradient centrifugation. Antheraea eucalypti cells were inoculated with the purified spores primed with 0.1 n KOH solution to start a continuous propagation of N. bombycis in cell culture. The first increase in the number of infected A. eucalypti cells was observed at 48 hr postinoculation, and it was caused by the secondary infective forms of N. bombycis. The secondary infective forms were produced during the course of sporoblast differentiation. The parasites in cell cultures divided synchronously until 36 hr postinoculation. Mature spores were observed initially 6 days postinoculation at 27°C. The infected cultures were subcultured extensively for more than 1 year with the addition of healthy A. eucalypti cells. |
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Keywords: | Antheraea eucalypti Urografin density gradient centrifugation cell culture |
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