Ovarian and haemolymph titres of ecdysteroid during the gonadotrophic cycle in Diploptera punctata |
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Authors: | Barbara Stay Lynda S Ostedgaard Stephen S Tobe Alain Strambi Eugene Spaziani |
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Institution: | 1. Department of Zoology, University of Iowa, Iowa City, Iowa 52242, U.S.A.;2. Department of Zoology, University of Toronto, Toronto M5S 1A1, Ontario, Canada;3. C.N.R.S. Institut de Neurophysiologie et Psychophysiologie, B.P. 71, 13277 Marseille, Cedex 9, France |
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Abstract: | The free (non-conjugated) ecdysteroid in the ovaries during the first gonadotrophic cycle of Diploptera punctata was identified as 20-hydroxyecdysone. The hormone, quantified by radioimmunoassay and by ultraviolet absorbance, was detectable in the ovary toward the end of vitellogenesis; the quantity increased rapidly during chorion formation. Ovaries with chorionated eggs contained 67 μg of 20-hydroxyecdysone per g fresh weight. The haemolymph free-ecdysteroid, not identified physicochemically, was quantified by radioimmunoassays. The highest concentration was observed at adult emergence; the titre declined between days 1–3 and then remained at a relatively constant level through oviposition (which occurs between day 7 and 8); titres in pregnant females were higher. Ovariectomized females exhibited the same pattern of ecdysteroid titres in the haemolymph as the sham operated controls throughout the period corresponding to the first gonadotrophic cycle. Thus the ovary may not be the only source of haemolymph ecdysteroid related to reproduction in adult females. |
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Keywords: | 20-Hydroxyecdysone ecdysteroids ovary haemolymph cockroach |
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