DNA binding spectrum of the carcinogen N-acetoxy-N-2-acetylaminofluorene significantly differs from the mutation spectrum |
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Authors: | Robert P.P. Fuchs |
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Affiliation: | Laboratoire de Biophysique, Institut de Biologie Moléculaire et Cellulaire du CRNS, 15 rue René Descartes, 67084 Strasbourg Cédex, France |
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Abstract: | The 3′ → 5′ exonuclease activity of bacteriophage T4 DNA polymerase is found to be blocked in the vicinity of the N-2-acetylaminofluorene (-AAF) adducts to DNA. This observation allowed us to determine the binding spectrum of the -AAF adducts along a given DNA sequence. The mutation spectrum in a forward mutation assay within this same sequence has been established. Comparison between the -AAF binding spectrum and the mutation spectrum shows that there is no direct correlation. |
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