Mitotic responses of the anterior pericardial wall of Biomphalaria glabrata (Mollusca) subjected to challenge |
| |
Authors: | John T Sullivan Thomas C Cheng Keith H Howland |
| |
Institution: | 1. Marine Biomedical Research Program Medical University of South Carolina P.O. Box 12559 (Fort Johnson) Charleston, South Carolina 29412 USA;2. Department of Anatomy (Cell Biology) Medical University of South Carolina P.O. Box 12559 (Fort Johnson) Charleston, South Carolina 29412 USA |
| |
Abstract: | Using light microscope autoradiography and electron microscopy we studied the effect of juvenile hormone III (JHIII) and β-ecdysone insect molting hormone (β-ecd) on the replication of Tipula iridescent virus (TIV) in suspension cultured cells of Estigmene acrea. JHIII at a concentration of 87.5 μg/ml completely inhibited viral DNA synthesis, but upon removal of JHIII, 3H]thymidine was incorporated into the cytoplasm as detected by autoradiography and virions in developmental stages from the same cell samples were-readily seen by electron microscopy. β-ecd at a concentration of 17.5 μg/ml, unlike JHIII, permitted viral DNA synthesis in the presence of the hormone although at a reduced level when compared to TIV-infected cells. But the presence of β-ecd seemed to prevent capsid formation, although islands similar in fine structure to those of viroplastic centers were seen by electron microscopy. Once β-ecd was removed from the medium, TIV-inoculated cells appeared to synthesize new virions in a normal pattern. Both hormones inhibited host cell DNA synthesis in noninfected cells. |
| |
Keywords: | anterior pericardial wall amoebocyte producing organ mitosis |
本文献已被 ScienceDirect 等数据库收录! |