A virus-inactivating protein isolated from the digestive juice of the silkworm, Bombyx mori |
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Authors: | Yuko Uchida Fumihiko Kawamoto Michio Himeno Keizo Hayashiya |
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Institution: | Faculty of Textile Science, Kyoto University of Industrial Arts and Textile Fibers, Matsugasaki, Kyoto 606, Japan;Department of Medical Zoology, Nagoya University School of Medicine, Nagoya 466, Japan;Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, Kyoto 606, Japan;Faculty of Textile Science, Kyoto University of Industrial Arts and Textile Fibers, Matsugasaki, Kyoto 606, Japan |
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Abstract: | A protein that can precipitate nuclear polyhedrosis virus (NPV) in vitro was isolated from the digestive juice of silkworm larvae (Bombyx mori) by the procedures of gel filtration and ion-exchange and hydroxylapatite column chromatography. The SDS-polyacrylamide gel electrophoretic and the ultracentrifugal analyses showed that the purified substance was a homogenous simple protein. The molecular weight of the purified protein was 27,000–28,000 and the sedimentation coefficient was 2.61 S. This protein had an additional activity to inactivate NPV of B. mori in vitro, somewhat analogous to serological neutralization by serum proteins. Electron microscope observations showed that amorphous materials could be found on the surface of envelopes and that the nucleocapsids disappeared. |
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Keywords: | insect virus nuclear polyhedrosis virus virus-inactivating protein precipitation of NPV To whom reprint requests should be addressed |
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