Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells |
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Authors: | Miyuki Kawakami Hiroshi Ishikawa Akira Tanaka Izumi Mataga |
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Affiliation: | 1.Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata,The Nippon Dental University,Niigata,Japan;2.Department of NDU Life Sciences, School of Life Dentistry,The Nippon Dental University,Tokyo,Japan;3.Department of Oral and Maxillofacial Surgery, Niigata Hospital,The Nippon Dental University,Niigata,Japan;4.Division of Cell Regeneration and Transplantation, Advanced Research Center, School of Life Dentistry at Niigata,The Nippon Dental University,Niigata,Japan |
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Abstract: | Atrophy or hypofunction of the salivary gland because of aging or disease leads to hyposalivation that affects patient quality of life by causing dry mouth, deterioration of mastication/deglutition, and poor oral hygiene status. Current therapy for atrophy or hypofunction of the salivary gland in clinical practice focuses on symptom relief using drugs and artificial saliva; therefore, there is still a need to develop new therapies. To investigate potential novel therapeutic targets, we induced the differentiation of salivary gland cells by co-culturing human adipose-derived stem cells isolated from buccal fat pads (hBFP-ASCs) with human salivary-gland-derived fibroblasts (hSG-fibros). We examined their potential for transplantation and tissue neogenesis. Following the culture of hBFP-ASCs and hSG-fibros, differentiated cells were transplanted into the submandibular glands of SCID mice, and their degree of differentiation in tissues was determined. We also examined their potential for functional tissue reconstitution using a three-dimensional (3D) culture system. Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo. Moreover, cell reconstituted glandular structures in the 3D culture system. In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues. |
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