Conidiation of Hirsutella thompsonii var. synnematosa in submerged culture |
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Authors: | AJ van Winkelhoff CW McCoy |
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Institution: | Department of Oral Microbiology, Free-University—Amsterdam, Amsterdam, The Netherlands;University of Florida, IFAS, Agricultural Research and Education Center, Lake Alfred, Florida 33850 USA |
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Abstract: | Fourteen monosporal isolates of Hirsutella thompsonii grew vegetatively in various liquid media producing typical phialidic-like conidiophores. Hirsutella thompsonii var. synnematosa from Ivory Coast (HtIC) was the only pathotype which produced true conidia in submerged culture. HtIC began producing conidia after 3 days incubation reaching a peak ranging from 6.8 × 105 to 9.7 × 107 conidia/ml between 6 and 11 days. A concentration of 10 g/liter of corn steep liquor and 0.2% Tween 80 were essential for maximum conidiation. Submerged conidia had a smooth but somewhat rugose conidial walls, whereas aerially formed conidia were distinctly verrucose. Germination of submerged conidia ranged from 5.2 to 12.9% and were virulent causing 32.5% infection to adult citrus rust mites when sprayed on citrus foliage at 1.2 × 109 conidia/ml. |
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Keywords: | conidiation submerged culture |
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