High frequency stable transformation ofArabidopsis thaliana by particle bombardment |
| |
Authors: | Misa Takahashi Hiromichi Morikawa |
| |
Affiliation: | (1) Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Kagamiyama, 739 Higashi-Hiroshima, Japan |
| |
Abstract: | Root explants ofArabidopsis thaliana ecotype C24 were bombarded with the plasmid pCH harboring the hygromycin phosphotransferase gene (hpt). A selection condition with post-bombardment culture of 3 days followed by culture with 20 mgl−1 hygromycin gave the highest yield of transformants. More than 44% of explant clumps formed transformant shoots. |
| |
Keywords: | Arabidopsis thaliana Hygromycin phosphotransferase gene (hpt) Particle bombardment Transgenic plant |
本文献已被 SpringerLink 等数据库收录! |