Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry |
| |
Authors: | Li Xiaochuan Yu Chen Cai Yongbao Liu Gangyi Jia Jingying Wang Yiping |
| |
Affiliation: | Department of Pharmacology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China; Graduate School of the Chinese Academy of Sciences, China. xchli@sibs.ac.cn |
| |
Abstract: | The six phenolic constituents are water-soluble components extracted from the Chinese medical herb danshen, the dried roots of Salvia miltiorrhiza Bunge (Labiatae). An liquid chromatography/tandem mass spectrometry (LC/MS/MS)-based method has been developed for the simultaneous quantification of six phenolic constituents of danshen (magnesium lithospermate B (MLB), rosmarinic acid (RA) and lithospermic acid (LA), caffeic acid (CAA), protocatechuic aldehyde (3,4-dihydroxybenzaldehyde, Pal), 3,4-dihydroxyphenyllactic acid (danshensu)) in human serum with chloramphenicol as internal standard. The serum samples were treated by special liquid-liquid extraction, and the analytes were determined using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode, with sufficient sensitivity to allow analysis of human serum samples generated following administration of a clinically relevant dose. Good linearity over the range 8-2048 ng/mL for six phenolic constituents was observed. The intra- and inter-day precisions (CV) of analysis were <13%, and the accuracy ranged from 88 to 116%. This quantitation method was successfully applied to a pharmacokinetic study of i.v. drip infusion of Danshen injection fluid in human. |
| |
Keywords: | Magnesium lithospermate B Rosmarinic acid Lithospermic acid Caffeic acid Protocatechuic aldehyde Danshensu Danshen Pharmacokinetics |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|