The gas-liquid cromatography with mass spectrometry for the identification of yeasts |
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Authors: | Paredes-Salido F Mira-Gutiérrez J Sasián-Macías P García-Martos P |
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Affiliation: | Departamento de Microbiología, Facultad de Medicina, C/ Padilla, n2-A, 11100-San Fernando, Cádiz, Espa?a. |
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Abstract: | Methods based on gas chromatography, have been used for identification of the yeasts. In order to know the value of the patterns obtained by this method, we have used this technique and mass spectrometry on 44 strains belonging to 16 genus and 21 species of collection yeasts, identifying the corresponding peaks to 22 fatty acids methyl esters by means of the reaction times of the corresponding standards and the confirmation of molecular weigh by mass spectrometry. The correlation coefficient was of 0.848965. The chromatographic technique seems of great utility for the determination of lipidotypes. |
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