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赤霉素对大岩桐花蕾离体培养直接再生花芽的影响(简报)
引用本文:庞基良 王利琳 胡江琴 梁海曼. 赤霉素对大岩桐花蕾离体培养直接再生花芽的影响(简报)[J]. 实验生物学报, 2004, 37(3): 241-246
作者姓名:庞基良 王利琳 胡江琴 梁海曼
作者单位:[1]杭州师范学院生命科学学院,杭州310036 [2]浙江大学西溪校区生命科学学院,杭州310012
摘    要:植物经过一定时期的营养生长(或感受外界信号)后,就能产生成花刺激物。成花刺激物被运输到茎尖,诱导发生一系列的反应。随后其分生组织在一定时期内处于一个相对稳定的状态,即成花决定态。植物成花决定态建立的过程称为成花决定。对成花决定的研究进行了许多年,但是其确切的机理仍不清楚.

关 键 词:大岩桐 赤霉素 花蕾 离体培养 再生花芽

Effect of gibberellin on direct regeneration of floral buds from young flower buds in Sinningia speciosa Hiern]
Ji Liang Pang,Li Lin Wang,Jiang Qin Hu,Hai Man Liang. Effect of gibberellin on direct regeneration of floral buds from young flower buds in Sinningia speciosa Hiern][J]. Acta Biologiae Experimentalis Sinica, 2004, 37(3): 241-246
Authors:Ji Liang Pang  Li Lin Wang  Jiang Qin Hu  Hai Man Liang
Affiliation:School of Life Sciences, Hangzhou Normal College, Hangzhou 310036.
Abstract:The phenomena of direct regeneration of floral buds on young flower bud and sepal cultures in vitro were observed in Sinningia speciosa Hiern. The gibberellin concentration in medium was very important for inducing the direct regeneration of floral buds. No direct floral bud was regenerated when the concentration of gibberellin (GA) supplemented in medium was equal to or less than 0.5 mg/L. The young floral buds and sepals inoculated on modified Murashige and Skoog (MS) medium supplemented with 1.0-1.5 mg/L GA and 0.1 mg/L 6-benzylaminopurine (BA) for 40 days produced floral buds directly on the surface of the sepals. There were two types of the floral buds regenerated directly: one was only with floral buds, which was up to 14.3%; the other was with both floral and vegetative buds, which was up to 28.6%. The effect of exogenous GA on the formation of floral buds in Sinningia cultured in vitro is discussed.
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