Somatic embryogenesis and plant regeneration in embryo cultures of Euterpe edulis mart. (palmae) |
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Authors: | Miguel Pedro Guerra Walter Handro |
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Affiliation: | (1) Plant Tissue Culture Laboratory, Department of Botany, Institute of Biosciences, University of São Paulo, 05499 São Paulo, Brazil;(2) Present address: Department of Phytotechny, UFSC, C.P. 476, 88049 Florianopolis, Brazil |
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Abstract: | The induction of somatic embryogenesis in embryo cultures of Euterpe edulis is described. The basal medium was composed of LS salts and Morel & Wetmore vitamins. Activated charcoal was added to prevent explant oxidation. 2,4-D higher than 50 mg/l was necessary for inducing embryogenesis which occurs 45–180 days after the start of cultures. Embryos arise directly from surface proliferating tissues on the matrix structure , without callus formation. The transfer of tissues with embryo clusters to medium with NAA plus 2iP, or without growth regulators, induces embryo development into plantlets. |
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Keywords: | LS: Linsmaier & Skoog 2,4-D: 2,4-dichlorophenoxiacetic acid 2iP: 2-isopentenyladenine NAA: naphthaleneacetic acid |
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