Colloidal gold staining and immunoprobing of proteins on the same nitrocellulose blot |
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Authors: | D Egger K Bienz |
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Institution: | Institute for Microbiology, University of Basel, Switzerland. |
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Abstract: | Proteins blotted onto nitrocellulose can be stained with colloidal gold. They retain their immunoreactivity so that the stained blot can be probed with appropriate antibodies. The resulting antigen-antibody reaction is made visible by a peroxidase-coupled antispecies antibody. The blue peroxidase reaction product contrasts well with the red-stained protein pattern and allows an easy documentation by black-and-white photography. |
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