Structural and functional characterization of osmotically inducible protein C (OsmC) from Thermococcus kodakaraensis KOD1 |
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Authors: | Park Seong-Cheol Pham Bang Phuong Van Duyet Le Jia Baolei Lee Sangmin Yu Rui Han Sang Woo Yang Jae-Kyung Hahm Kyung-Soo Cheong Gang-Won |
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Institution: | Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, Jinju, 660-701, Republic of Korea. |
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Abstract: | Osmotically inducible protein C (OsmC) is involved in the cellular defense mechanism against oxidative stress caused by exposure to hyperoxides or elevated osmolarity. OsmC was identified by two-dimensional electrophoresis (2DE) analysis as a protein that is overexpressed in response to osmotic stress, but not under heat and oxidative stress. Here, an OsmC gene from T. kodakaraensis KOD1 was cloned and expressed in Escherichia coli. TkOsmC showed a homotetrameric structure based on gel filtration and electron microscopic analyses. TkOsmC has a significant peroxidase activity toward both organic and inorganic peroxides in high, but not in low temperature. |
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