The ectomycorrhizal morphotype Pinirhiza sclerotia is formed by Acephala macrosclerotiorum sp. nov., a close relative of Phialocephala fortinii |
| |
Authors: | Babette Münzenberger Ben Bubner Jens Wöllecke Thomas N Sieber Robert Bauer Matthias Fladung Reinhard F Hüttl |
| |
Institution: | 1. Institute of Landscape Matter Dynamics, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Stra?e 84, 15374, Müncheberg, Germany 2. Brandenburg University of Technology, Chair of Soil Protection and Recultivation, P.O. Box 101344, 03013, Cottbus, Germany 4. ETH Zürich, Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, CH-8092, Zürich, Switzerland 5. Eberhard-Karls-University Tübingen, Botanical Institute, Systematic Botany and Mycology, Auf der Morgenstelle 1, 72076, Tübingen, Germany 6. Johann Heinrich von Thünen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstra?e 2, 22927, Grosshansdorf, Germany 3. German Research Centre of Geosciences Potsdam (GFZ), Telegrafenberg, 14473, Potsdam, Germany
|
| |
Abstract: | Relatively few ectomycorrhizal fungal species are known to form sclerotia. Usually, sclerotia are initiated at the extraradical
mycelium. In this study, we present anatomical and ultrastructural evidence for the formation of sclerotia directly in the
hyphal mantle of the mycorrhizal morphotype Pinirhiza sclerotia. A dark-pigmented fungal strain was isolated from Pinirhiza sclerotia and identified by molecular tools as Acephala macrosclerotiorum sp. nov., a close relative of Phialocephala fortinii s.l. As dark septate fungi are known to be mostly endophytic, resyntheses with Pinus sylvestris and A. macrosclerotiorum as well as Populus tremula × Populus tremuloides and A. macrosclerotiorum or P. fortinii s.l. were performed under axenic conditions. No mycorrhizas were found when hybrid aspen was inoculated with A. macrosclerotiorum or P. fortinii. However, A. macrosclerotiorum formed true ectomycorrhizas in vitro with P. sylvestris. Anatomical and ultrastructural features of this ectomycorrhiza are presented. The natural and synthesized ectomycorrhizal
morphotypes were identical and characterized by a thin hyphal mantle that bore sclerotia in a later ontogenetic stage. The
Hartig net was well-developed and grew up to the endodermis. To our knowledge, this is the first evidence at the anatomical
and ultrastructural level that a close relative of P. fortinii s.l. forms true ectomycorrhizas with a coniferous host. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|