A compact phage display human scFv library for selection of antibodies to a wide variety of antigens |
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Authors: | Potjamas Pansri Nanthnit Jaruseranee Kuntalee Rangnoi Peter Kristensen Montarop Yamabhai |
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Affiliation: | (1) School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand;(2) Department of Molecular Biology, University of Aarhus, Science Park, DK-8000 Aarhus C, Denmark |
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Abstract: | Background Phage display technology is a powerful new tool for making antibodies outside the immune system, thus avoiding the use of experimental animals. In the early days, it was postulated that this technique would eventually replace hybridoma technology and animal immunisations. However, since this technology emerged more than 20 years ago, there have only been a handful reports on the construction and application of phage display antibody libraries world-wide. |
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