Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato |
| |
| Authors: | Sheh May Tam Sompid Samipak Anne Britt Roger T Chetelat |
| |
| Institution: | (1) Present address: School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia;(2) Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;(3) Department of Plant Biology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA;(4) Present address: Department of Genetics, Kasetsart University, Bangkok, 10900, Thailand; |
| |
| Abstract: | DNA mismatch repair proteins play an essential role in maintaining genomic integrity during replication and genetic recombination.
We successfully isolated a full length MSH2 and partial MSH7 cDNAs from tomato, based on sequence similarity between MutS
and plant MSH homologues. Semi-quantitative RT-PCR reveals higher levels of mRNA expression of both genes in young leaves
and floral buds. Genetic mapping placed MSH2 and MSH7 on chromosomes 6 and 7, respectively, and indicates that these genes
exist as single copies in the tomato genome. Analysis of protein sequences and phylogeny of the plant MSH gene family show
that these proteins are evolutionarily conserved, and follow the classical model of asymmetric protein evolution. Genetic
manipulation of the expression of these MSH genes in tomato will provide a potentially useful tool for modifying genetic recombination
and hybrid fertility between wide crosses. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
