Homocysteine is a potent inhibitor of human tumor cell gelatinases |
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Authors: | Chavarría Teresa Rodríguez-Nieto Salvador Sánchez-Jiménez Francisca Quesada Ana R Medina Miguel A |
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Institution: | Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, E-29071 Málaga, Spain. |
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Abstract: | Extracellular matrix-degrading gelatinases are mainly involved in tumor invasion and metastasis. Previous experimental data from our group and others suggested that homocysteine could have a potential modulatory role on the proteolytic balance at the extracellular matrix. Therefore, we studied the effects of homocysteine on extracellular matrix-degrading proteases using model human tumor cell lines and a combination of in vitro fluorogenic assay and zymographic techniques. Homocysteine is shown to be the thiol compound with the most potent inhibitory activity on matrix metalloproteinase 9. Zymographies reveal that matrix metalloproteinase 2 is, at least, as sensitive to inhibition by homocysteine as matrix metalloproteinase 9 is. This study opens new ways to the potential pharmacological use of thiol compounds. |
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Keywords: | Homocysteine Matrix metalloproteinases Gelatinases HT-1080 cells U2-OS cells |
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