Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles |
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| Authors: | Lucci Carolina M Kacinskis Mirella A Rumpf Rodolfo Báo Sônia N |
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| Institution: | 1. Lab. Microscopia Electrônica, Depto. Biologia Celular, IB, UnB, 70910-900 Brası́lia, DF, Brazil;2. EMBRAPA Genetic Resources and Biotechnology, Brası́lia, DF, Brazil;1. Centro de Biotecnología de la Reproducción (BIOREN-CEBIOR), Facultad de Medicina, Universidad de La Frontera, Temuco, Chile;2. Departamento de Ciencias Básicas, Universidad de La Frontera, Temuco, Chile;3. Departamento de Ciencias Agronómicas y Recursos Naturales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco, Chile;4. Centro ANDROGEN, La Coruña, Spain;5. Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain;6. Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile;1. Silkworm Resource Division, Institute of Genetic Resources, Kyushu University Graduate School of Bio Resources and Bioenvironmental Science, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;2. Graduate School of Agricultural and Life Science, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan;3. Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan;1. Division of Endocrinology, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois;2. Division of Urology, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois;3. Division of Hematology Oncology, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois;4. Division of Pediatric Surgery, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois;5. Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois;6. Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois;7. Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, Illinois;8. Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, Illinois;9. Department of Obstetrics and Gynecology, Northwestern University Feinberg School of Medicine, Chicago, Illinois;1. Department of Thoracic and Cardiovascular Surgery and Angiology, Limoges University Hospital Center, 87000 Limoges, France;2. Department of Pharmacology, Toxicology and Pharmacovigilance, INSERM UMR S-850, Limoges, France;3. Equipe de Recherche Médicale Appliquée (ERMA), University of Limoges, 87000, France;4. Department of Anesthesia and Intensive Care, Limoges University Hospital Center, 87000 Limoges, France;5. Department of Biochemistry and Molecular Genetics, Limoges University Hospital Center, 87000 Limoges, France;6. Department of Pathological Anatomy, Limoges University Hospital Center, 87000 Limoges, France;7. Department of Radiology and Medical Imaging, Limoges University Hospital Center, 87000 Limoges, France;8. Research and Analysis Laboratory of Haute-Vienne, 87000 Limoges, France;9. Department of Obstetric and Gynecology, Limoges University Hospital Center, 87000 Limoges, France |
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| Abstract: | The maintenance of follicle quality during the transportation of ovaries is essential for the successful cryopreservation and in vitro development of preantral follicles. The objective of this study was to evaluate the effect of cooling ovarian tissue on the conservation of zebu cow preantral follicles. Ovarian pieces were immersed in saline or coconut water (CW) solutions and maintained at 4 or 20 degrees C for 6, 12, or 18 h. Preantral follicles were evaluated by histology and transmission electron microscopy. Storage of ovarian pieces at 20 degrees C for 12 or 18 h significantly reduced the percentage of morphologically normal follicles compared to controls. In contrast, conservation at 4 degrees C for up to 18 h and at 20 degrees C for up to 6 h kept the percentage of normal follicles similar to controls. However, the type of solution that the ovaries were immersed in had little effect on the results. Decreased cellular metabolism probably accounted for better preservation of preantral follicles at 4 degrees C. In conclusion, zebu cow ovaries were successfully stored at 4 degrees C for up to 18 h with no morphological damage to preantral follicles. However, at 20 degrees C, ovaries could only be stored for 6 h. |
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