Adhesion of lily pollen tubes on an artificial matrix |
| |
Authors: | Guang Yuh Jauh Kathleen J Eckard Eugene A Nothnagel E M Lord |
| |
Institution: | (1) Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA, Tel. 1-909-7874441; Fax +1-909-7874437 e-mail lord@citrus.ucr.edu, US |
| |
Abstract: | We proposed that pollination in lily is a case of cell adhesion and cell movement, but experimental evidence for the adhesion
event is lacking. In this study, we developed an artificial extracellular matrix that mimics the in vivo lily stylar transmitting
tract. This artificial matrix was created by applying the transmitting tract exudate extracted from lily styles onto a nitrocellulose
membrane. When in vitro-grown pollen tubes were applied to the matrix, they adhered by their tips to the area of the stylar
exudate which is rich in arabinogalactan proteins. Once they adhered, they grew on the in vitro artificial matrix at rates
faster than normal. This is the first experimental evidence demonstrating the adhesion of in vitro-grown pollen tubes, an
event that has been described as common in vivo. The adhesion event is stylar exudate specific, concentration dependent, and
is affected by the developmental age of the pollen tube. This bioassay for pollen tube adhesion will be used to isolate the
adhesive molecules from the stylar exudate.
Received: 9 December 1996 / Revision accepted: 5 May 1997 |
| |
Keywords: | Adhesion assay Artificial plant extracellular matrix Lilium longiflorum Thunb Pollen tube Stylar exudate |
本文献已被 SpringerLink 等数据库收录! |
|