Desiccated doubled-haploid embryos obtained from microspore culture of barley cv. Igri |
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Authors: | A B Ryan A M Castillo M P Vallés J M Sanz L Cistué |
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Institution: | (1) Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Apartado 202, E-50080 Zaragoza, Spain e-mail: lcistue@eead.csic.es Fax: +34-976-575620, ES |
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Abstract: | Barley microspore-derived doubled-haploid embryos have been produced in vitro. The development of embryo desiccation technology will allow long-term storage, germplasm preservation and low delivery cost.
Treatment of the microspore-derived embryos was essential to induce desiccation tolerance and to arrest further development
and plant regeneration. At the concentrations used, a treatment with trehalose was more efficient than with sucrose, and mannitol
was harmful to the embryos. Up to 80% of the desiccated embryos produced complete green plants when transferred to regeneration
medium, by the application of a 0.6 m trehalose or a 10–5 m abscisic acid treatment to the embryos in the culture induction medium. The morphology of these plants was similar to plants
produced directly from non-desiccated embryos.
Received: 28 September 1998 / Revision received: 27 November 1998 / Accepted: 5 January 1999 |
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Keywords: | Barley Desiccation Embryos Doubled haploids Microspores |
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