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Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco |
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| Authors: | Qiu-Li Li Xiao-Rong Gao Xiao-Hong Yu Xing-Zhi Wang Li-Jia An |
| Institution: | (1) Insitute of Life Science, Liaoning Normal University, Dalian, 116029, P.R. China;(2) Department of Bioengineering, Dalian University of Technology, Dalian, 116012, P.R. China;(3) 111 Koshland Hall, Department of Plant Microbial & Biology, University of California at Berkeley, Berkeley, CA 94720-3102, USA;(4) Department of Biology, North east Normal University, Changchun, 130024, P.R. China |
| Abstract: | cDNA encoding betaine aldehyde dehydrogenase (BADH) from the halophyte Suaeda liaotungensis has been cloned, sequenced and expressed in tobacco (Nictiana tabacum 89). The full-length cDNA is 1506 base pairs (bp) long and encodes a 502 amino-acid polypeptide. The cDNA fragment coding for the mature enzyme was cloned into vector pCAMBIA-1301 for expression in tobacco. Southern blotting analysis showed that that the Badh gene was integrated into the genome of tobacco. Tobacco expressing BADH survived on MS medium containing 200 mM NaCl, whereas the untransformed plants turned yellow after about 20 d and died. |
| Keywords: | betaine aldehyde dehydrogenase gene-cloning salt tolerance Suaeda liaotungensis transgenic tobacco |
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