Influence de la température sur quelques activités enzymatiques chez Palaemon serratus |
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Authors: | Joël Trellu Hubert J. Ceccaldi |
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Affiliation: | Ecole Pratique des Hautes Etudes, Laboratoire de Biochimie et Ecologie des Invertébrés marins, Station marine d''Endoume, 13007 Marseille, France |
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Abstract: | Variation in Esterase 2 C activities, involving the hydrolysis of 2-carboxylic esters, α-glucosidase acetyl-glucosaminidase and alkaline and acid phosphotases, in the hepatopancreas and the abdominal muscle of Palaemon serratus was examined by polyacrylamide gradient gel electrophoresis. Soluble proteins were measured in the hepatopancreas and the abdominal muscle, and trypsin and chymotrypsin activities in the hepatopancreas. The activities and the isoenzymatic variations in shrimps acclimated at 5 different temperatures (between 14 and 30°) were compared and the molecular weight of each isozyme evaluated. It was found that: (a) the concentrations of soluble proteins decrease in the hepatopancreas between 18 and 30°, but remain unchanged in the abdominal muscle; (b) esterase and phosphatase activities increase with temperature but in a more or less random manner, according to the isozyme under consideration; (c) glycosidase activities increase with temperature; and (d) trypsin activity varies in an inverse relation to chymotrypsin activity. |
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Keywords: | Crustacea Decapoda zymograms temperature hepatopancreas abdominal muscle ecology |
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