Changes in composition and sulfation patterns of glycoaminoglycans in renal cell carcinoma |
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Authors: | Ebru Ucakturk Orkun Akman Xiaojun Sun Anil Dolgun " target="_blank">Fuming Zhang " target="_blank">Robert J Linhardt |
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Institution: | 1.Department of Analytical Chemistry, Faculty of Pharmacy,Hacettepe University,S?hh?ye,Turkey;2.Department of Pathology,Hacettepe University School of Medicine,S?hh?ye,Turkey;3.Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute,Troy,USA;4.Department of Biology, Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute,Troy,USA;5.Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute,Troy,USA;6.Department of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute,Troy,USA;7.Department of Biostatistics, Faculty of Medicine,Hacettepe University,S?hh?ye,Turkey |
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Abstract: | Glycosaminoglycans (GAGs) are heterogeneous, linear, highly charged, anionic polysaccharides consisting of repeating disaccharides units. GAGs have some biological significance in cancer progression (invasion and metastasis) and cell signaling. In different cancer types, GAGs undergo specific structural changes. In the present study, in depth investigation of changes in sulfation pattern and composition of GAGs, heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate and hyaluronan (HA) in normal renal tissue (NRT) and renal cell carcinoma tissue (RCCT) were evaluated. The statistical evaluation showed that alteration of the HS (HSNRT = 415.1 ± 115.3; HSRCCT = 277.5 ± 134.3), and CS (CSNRT = 35.3 ± 12.3; CSRCCT = 166.7 ± 108.8) amounts (in ng/mg dry tissue) were statistically significant (p < 0.05). Sulfation pattern in NRT and RCCT was evaluated to reveal disaccharide profiles. Statistical analyses showed that RCCT samples contain significantly increased amounts (in units of ng/mg dry tissue) of 4SCS (NRT = 25.7 ± 9.4; RCCT = 117.1 ± 73.9), SECS (NRT = 0.7 ± 0.3; RCCT = 4.7 ± 4.5), 6SCS (NRT = 6.1 ± 2.7; RCCT = 39.4 ± 34.7) and significantly decreased amounts (in units of ng/mg dry tissue) of NS6SHS (RCCT = 28.6 ± 6.5, RCCT = 10.2 ± 8.0), NS2SHS (RCCT = 44.2 ± 13.8; RCCT = 27.2 ± 15.0), NSHS (NRT = 68.4 ± 15.8; RCCT = 50.4 ± 21.2), 2S6SHS (NRT = 1.0 ± 0.4; RCCT = 0.4 ± 0.3), and 6SHS (NRT = 60.6 ± 17.5; RCCT = 24.9 ± 12.3). If these changes in GAGs are proven to be specific and sensitive, they may serve as potential biomarkers in RCC. Our findings are likely to help us to show the direction for further investigations to be able to bring different diagnostic and prognostic approaches in renal tumors. |
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