Selective binding of antimicrobial porphyrins to the heme‐receptor IsdH‐NEAT3 of Staphylococcus aureus |
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Authors: | Nhuan T Vu Yoshitaka Moriwaki Jose M M Caaveiro Tohru Terada Hiroshi Tsutsumi Itaru Hamachi Kentaro Shimizu Kouhei Tsumoto |
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Institution: | 1. Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, , Tokyo, 108‐8639 Japan;2. Department of Biology, College of Sciences, Cantho University, , Cantho,, Vietnam;3. Department of Biotechnology, The University of Tokyo, , Tokyo, 113‐8657 Japan;4. Agricultural Bioinformatics Unit, School of Agricultural and Life Sciences, The University of Tokyo, , Tokyo, 113‐8657 Japan;5. Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, , Kyoto, 615‐8510 Japan;6. Department of Medical Genome Sciences, School of Frontier Sciences, , Kashiwa, 277‐8562 Japan;7. Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, , Tokyo, 113‐8656 Japan |
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Abstract: | The Isd (iron‐regulated surface determinant) system of the human pathogen Staphylococcus aureus is responsible for the acquisition of heme from the host organism. We recently reported that the extracellular heme receptor IsdH‐NEAT3 captures and transfers noniron antimicrobial porphyrins containing metals in oxidation state (III). However, it is unclear if geometric factors such as the size of the metal (ionic radius) affect binding and transfer of metalloporphyrins. We carried out an ample structural, functional, and thermodynamic analysis of the binding properties of antimicrobial indium(III)‐porphyrin, which bears a much larger metal ion than the iron(III) of the natural ligand heme. The results demonstrate that the NEAT3 receptor recognizes the In(III)‐containing PPIX in a manner very similar to that of heme. Site‐directed mutagenesis identifies Tyr642 as the central element in the recognition mechanism as suggested from the crystal structures. Importantly, the NEAT3 receptor possesses the remarkable ability to capture dimers of metalloporphyrin. Molecular dynamics simulations reveal that IsdH‐NEAT3 does not require conformational changes, or large rearrangements of the residues within its binding site, to accommodate the much larger (heme)2 ligand. We discuss the implications of these findings for the design of potent inhibitors against this family of key receptors of S. aureus. |
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Keywords: | metal recognition metalloporphyrin antimicrobial compound Isd system isothermal titration calorimetry molecular dynamics simulations protein– heme interaction |
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