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A general synthetic method toward uridylylated picornavirus VPg proteins |
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| Authors: | Gerbrand J van der Heden van Noort Catherine H Schein Herman S Overkleeft Gijsbert A van der Marel Dmitri V Filippov |
| Institution: | 1. Leiden Institute of Chemistry, Leiden University, , RA, 2300 Leiden, The Netherlands;2. Department of Microbiology and Immunology, University of Texas Medical Branch, , Galveston, TX, 77555‐0857 USA |
| Abstract: | Small proteins called viral protein genome‐linked (VPg), attached to the 5′‐end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre‐uridylylated tyrosine building block in an on‐line solid phase‐based approach. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd. |
| Keywords: | viral nucleoprotein uridylylation post‐translational modification on‐line peptide synthesis |