Proteome profiling of peripheral mononuclear cells from human blood |
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Authors: | Giuseppina Maccarrone Christiane Rewerts Maria Lebar Christoph W. Turck Daniel Martins‐de‐Souza |
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Affiliation: | 1. Max Planck Institute for Psychiatry, Proteomics and Biomarkers, , Munich, Germany;2. Dept of Psychiatry, Ludwig Maximilians University (LMU), , Munich, Germany;3. Lab. de Neurociências (LIM‐27), Inst. de Psiquaitria, Faculdade de Medicina da Universidade de Sao Paulo, , S?o Paulo, Brazil |
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Abstract: | Peripheral blood mononuclear cells (MNCs) are accessible through blood collection and represent a useful source for investigations on disease mechanisms and treatment response. Aiming to build a reference proteome database, we generated three proteome data sets from MNCs using a combination of SDS‐PAGE and nanoflow LC‐MS. Experiments were performed in triplicates and 514 unique proteins were identified by at least two non‐redundant peptides with 95% confidence for all replicates. Identified proteins are associated with a range of dermatologic, inflammatory and neurological conditions as well as molecular processes, such as free radical scavenging and cellular growth and proliferation. Mapping the MNC proteome provides a valuable resource for studies on disease pathogenesis and the identification of therapeutic targets. |
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Keywords: | Biomarkers Biomedicine Blood Diseases Mononuclear cells Shotgun |
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