Interaction of colchicine with human serum albumin investigated by spectroscopic methods |
| |
Authors: | Hu Yan-Jun Liu Yi Zhao Ru-Ming Qu Song-Sheng |
| |
Institution: | College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China. |
| |
Abstract: | We investigated the interaction between colchicine and human serum albumin (HSA) by fluorescence and UV-vis absorption spectroscopy. In the mechanism discussion, it was proved that the fluorescence quenching of HSA by colchicine is a result of the formation of colchicines-HSA complex; van der Waals interactions and hydrogen bonds play a major role in stabilizing the complex. The modified Stern-Volmer quenching constant K(a) and corresponding thermodynamic parameters deltaH, deltaG, deltaS at different temperatures were calculated. The distance r between donor (Trp214) and acceptor (colchicine) was obtained according to fluorescence resonance energy transfer (FRET). |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|