Indirect measurements of differential gene expression with cDNA microarrays |
| |
Authors: | Belbin Thomas J Gaspar John Haigentz Missak Perez-Soler Roman Keller Steven M Prystowsky Michael B Childs Geoffrey Socci Nicholas D |
| |
Affiliation: | Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. |
| |
Abstract: | The use of universal RNA reference sets is an increasingly common approach to molecular classification studies with cDNA microarrays. Here we evaluated the reliability of indirect measurements of fluorescence ratios with a common RNA reference as a means of identifying differentially expressed genes. Comparisons of direct and indirect measures of differential gene expression showed a strong overall correlation in fluorescence ratio measurements but also a high degree of false positives in our indirect measurements. These results indicated that the application of more stringent ratio filters may be required when assessing differential gene expression utilizing a common RNA reference in classification studies. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |