Presence of dynorphin-like immunoreactivity in rat pituitary gland and hypothalamus |
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Authors: | K Nakao T Yoshimasa S Oki I Tanaka Y Nakai M Wakimasu M Fujino H Imura |
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Affiliation: | 1. Second Division, Department of Internal Medicine, Kyoto University School of Medicine, Sakyo-ku, Kyoto 606, Japan;2. Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka 532, Japan |
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Abstract: | Using a highly specific and sensitive radioimmunoassay for dynorphin(1-13), dynorphin-like immunoreactivity (dynorphin-LI) was detected in rat pituitary and hypothalamus. Gel chromatographic studies on Sephadex G-50 revealed three components of dynorphin-LI with molecular weights of approximately 7500-9500 (big dynorphin), 3500-5500 (intermediate dynorphin) and 1600-1900 (small dynorphin), the latter of which eluted at the same position as authentic dynorphin contamination in porcine ACTH extracts (Sigma). Dynorphin-LI in rat anterior pituitary existed mainly as big dynorphin, whereas dynorphin-LI in rat intermediate-posterior pituitary and hypothalamus eluted mainly at the position of authentic small dynorphin. |
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Keywords: | dynorphin Leu-enkephalin opioid peptide radioimmunoassay pituitary hypothalamus precursor |
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