The influence of intracellular pH of spores on citric acid production by Aspergillus niger |
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Affiliation: | Boris Kidrič Institute of Chemistry, Ljubljana, Yugoslavia |
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Abstract: | The spores with different intracellular pH values were produced by cultivating Aspergillus niger on potato-dextrose agar at different pHe values. High citric acid production is obtained using spores with the highest initial pH values (pHi) around 7.5. After a drop of intracellular pH during germination of spores of about 0.7 units, the conditions for highest rate of metabolic flow through the glycolytic pathway were achieved only by the mycelium grown out of spores with the highest pHi, since a very narrow pH optimum of 6-phosphofructokinase activity has been found around 7.5. |
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Keywords: | Aspergillus niger Intracellular pH 6-Phosphofructokinase Citric acid |
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