Odorant- and guanine nucleotide-stimulated phosphoinositide turnover in olfactory cilia |
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| Authors: | T Huque R C Bruch |
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| Affiliation: | 1. Maryland Psychiatric Research Center, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD, USA;2. Department of Cellular and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA;3. Laboratorio de Neurobioquimica y Conducta, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, S.S.A. Ciudad de México, Mexico;4. Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA;1. Environmental Remediation Research Group, Korea Institute of Science and Technology (KIST), Gangneung Institute, Gangneung 210-340, South Korea;2. Department of Chemistry, Seoul Women’s University, Seoul 139-774, South Korea;3. Natural Medicine Center, Korea Institute of Science and Technology (KIST), Gangneung Institute, Gangneung 210-340, South Korea;1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China;2. University of Science and Technology of China, Hefei, Anhui 230026, China;3. University of Chinese Academy of Sciences, Beijing 100039, China;1. Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
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| Abstract: | Isolated olfactory cilia from the channel catfish (Ictalurus punctatus) exhibited phosphatidylinositol-4,5-bisphosphate phosphodiesterase (E.C.3.1.4.11) activity. The phosphodiesterase activity was stimulated in the presence of an odorant for the catfish, namely the amino acid L-alanine. The enzyme activity was also stimulated in the presence of GTP and its nonhydrolyzable analogues. The activation of the phosphodiesterase by guanine nucleotides, in combination with the identification of guanine nucleotide-binding protein(s) in the isolated cilia, indicate the probable participation of a guanine nucleotide-binding protein in stimulation of phosphoinositide turnover in the olfactory receptor neuron. |
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